Oxford Nanopore Launches Flongle for Rapid, Smaller DNA/RNA Sequencing Tests in Any Environment

Oxford, England (ots/PRNewswire) - Oxford Nanopore has today made
Flongle starter packs available to purchase
https://store.nanoporetech.com/flongle.html, after initial testing in
an early-access programme. Flongle introduces a new paradigm of
smaller, on-demand DNA or RNA sequencing tests, with the potential to
transform a range of applications where rapid insights are required
at low cost.

Oxford Nanopore's goal is to enable the analysis of any living
thing, by anyone, anywhere. Flongle is designed to bring this goal
closer and to also allow low-cost sequencing at any time. With its
compact format, Flongle opens up possibilities of on-demand testing
in a broad range of locations, from scientific laboratories to farms
and factories, healthcare settings, outdoor environments or
classrooms.

Flongle is an adapter for the portable MinION and desktop GridION
X5 sequencing devices. It uses the same core nanopore sequencing
technology as other Oxford Nanopore devices, producing real-time
data, direct sequencing and long reads. The small Flongle flow cells
can currently produce as much as 1.8 Gb of sequence data with
headroom for more than 3 Gb. This enables many sequencing experiment
types, including small-genome sequencing, targeted panels/amplicon
sequencing, metagenomics for the identification of viruses, bacteria
or characterisation of microbiomes, or species ID. It can also be
used for quality control for larger nanopore experiments.

At $90 per flow cell, Flongle allows on-demand sequencing at an
affordable price point, without needing to wait for the large batches
of samples that are often required to achieve low per-test costs on
traditional sequencing devices. The workflow is fast and simple, with
library preparation in as little as ten minutes and real-time data
analysis.

Dr Matt Loose, from DeepSeq, School of Life Sciences, University
of Nottingham, has been testing the Flongle as part of an early
access programme. "We've been using Flongle for testing and
developing new protocols such as CRISPR/Cas9 selection for targeting
select regions of the human genome, as well as working up new samples
before running at scale on PromethION. We've been very happy with
throughput and yield on early access flow cells and are looking
forward to incorporating production-ready Flongle into our workflows.
It will be really interesting to see how users apply Flongle in the
field."

https://twitter.com/mattloose/status/1067115112220295168

https://twitter.com/mattloose/status/1053307734802776070

Miten Jain, from the University of California, Santa Cruz, has
also been trialling Flongle: "Flongle-based sequencing is the best
way to check a nanopore library. At UC Santa Cruz, we now routinely
sequence native RNA and genomic DNA libraries on a Flongle Flow Cell
before scaling them to our PromethION. We have observed that
Flongle-based sequencing is a good predictor of library quality and
its performance on the PromethION. Flongle also permits experimental
tests at a more affordable scale and will be an integral part of our
nanopore work going forward."

Dr Justin O'Grady is a translational researcher from University of
East Anglia, UK. He has been developing nanopore-based research
methods for the rapid metagenomic characterisation of pathogens in
clinical samples from patients with pneumonia or urinary tract
infections. "I am excited about how Flongle can help identify
pathogens and antimicrobial resistance more quickly, and how such
technologies could be used in settings much closer to patients in the
future."

https://twitter.com/Justin_OGrady/status/1055855306754924544

Flongle starter packs are now available in store. These starter
packs include the Flongle adapter and users can choose to run these
on their MinION or GridION devices. Users will be able to purchase
additional flow cells for $90 each. Protocols are being made
available and use the current preparation kits already compatible
with MinION, GridION and PromethION.

Oxford Nanopore is now taking pre-orders for the MinION Mk1C
https://nanoporetech.com/products/minion-mk1c, which combines the
real-time, portable sequencing of MinION with powerful computing and
a high-resolution touchscreen. This device will be compatible with
Flongle enabling rapid, field-based analyses; more information will
be provided soon.

Video -
https://mma.prnewswire.com/media/834428/Oxford_Nanopore_Flongle.mp4

Photo -
https://mma.prnewswire.com/media/834426/Oxford_Nanopore_Flongle.jpg

Contact: Zoe McDougall, media@nanoporetech.com

ots Originaltext: Oxford Nanopore Technologies
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